Author Correction for Hyman et al., Evaluation of a Fully Automated Research Prototype for the Immediate Identification of Microorganisms from Positive Blood Cultures under Clinical Conditions

نویسندگان

  • Jay M. Hyman
  • John D. Walsh
  • Christopher Ronsick
  • Mark Wilson
  • Kevin C. Hazen
  • Larisa Borzhemskaya
  • John Link
  • Bradford Clay
  • Michael Ullery
  • Mirta Sanchez-Illan
  • Steven Rothenberg
  • Ron Robinson
  • Alex van Belkum
  • W. Michael Dunne
چکیده

UNLABELLED A clinical laboratory evaluation of an intrinsic fluorescence spectroscopy (IFS)-based identification system paired to a BacT/Alert Virtuo microbial detection system (bioMérieux, Inc., Durham, NC) was performed to assess the potential for fully automated identification of positive blood cultures. The prototype IFS system incorporates a novel method combining a simple microbial purification procedure with rapid in situ identification via spectroscopy. Results were available within 15 min of a bottle signaling positive and required no manual intervention. Among cultures positive for organisms contained within the database and producing acceptable spectra, 75 of 88 (85.2%) and 79 of 88 (89.8%) were correctly identified to the species and genus level, respectively. These results are similar to the performance of existing rapid methods. IMPORTANCE A fully automated research platform was developed to identify microbial growth from positive blood cultures in <15 min. Because of the automated format, results can be generated during all shifts, with or without staffing, which in turn could promote more timely administration of target antimicrobial therapy.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Platelet Septic Transfusion Reactions in Patients With Hemato-Oncological Diseases

Background & Objective: Bacterial, contamination of blood components are a significant risk for transfusion reactions. Inherently, platelet concentrates (PCs) are vulnerable to bacterial contamination, due to the storage condition of processed PCs at room temperature, which provide very suitable conditions for the proliferation of microorganisms....

متن کامل

آلودگی کشت خون و انواع میکروارگانیزم های مثبت واقعی و کاذب در بیماران بستری در بیمارستان بوعلی سینا قزوین

Introduction: Diagnosis of infection based on blood culture alone is not a suitable method, but it is important to understand the clinical diagnosis for interpreting blood cultures. The goal of this study was to determine blood culture contamination and the type of microorganisms in false and real positive cases in patients admitted at Avicenna Hospital. Methods: This cross sectional study was...

متن کامل

Large-Scale Expansion of Human Embryonic and Induced Pluripotent Stem Cells for Cell Therapy Applications

Successful isolation, derivation and culturing of human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem (hiPSCs) cells in laboratory scale has opened new horizones for cell therapy applications such as tissue engineering and regenerative medicine. However, most of the cell therapy protocols using these unique cells require large number of ...

متن کامل

Rapid Intrinsic Fluorescence Method for Direct Identification of Pathogens in Blood Cultures

UNLABELLED A positive blood culture is a critical result that requires prompt identification of the causative agent. This article describes a simple method to identify microorganisms from positive blood culture broth within the time taken to perform a Gram stain (<20 min). The method is based on intrinsic fluorescence spectroscopy (IFS) of whole cells and required development of a selective lys...

متن کامل

Determination of Frequency of Positive Blood Culture Samples and Antibiotic Resistance Pattern of Isolated Bacteria from Patients Suspected of Infectious Diseases Admitted to Imam Khomeini Hospital in Ahvaz in 2013-2016

Introduction: The invasion of microorganisms into the bloodstream and spread to different parts of body can cause disruption the functions of vital organs and even death. This study aimed to evaluation of positive blood cultures in patients suspected to septicemia admitted to the Imam Khomeini Hospital in 2013-2016. Materials and Methods:  In this study, blood samples of patients were cultured ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2016